NAD+: Dosage & Administration

NAD+ dosing varies significantly by route of administration, with injectable forms providing direct bioavailability and oral precursors requiring enzymatic conversion.

Route	Dose	Frequency	Primary Use
Subcutaneous injection	100–500 mg	Daily or 2–3× weekly	Direct NAD+ repletion, anti-aging protocols
IV infusion	250–750 mg	1–2× weekly (clinic)	Rapid NAD+ loading, neurodegenerative research
Oral NMN	250–1000 mg	Daily	Maintenance, convenience
Oral NR	300–1000 mg	Daily	Maintenance, most clinical data

Subcutaneous NAD+ protocols typically start at 100–200 mg daily, with some protocols increasing to 500 mg. The higher doses are associated with more injection site discomfort.

Injectable NAD+ is supplied as a lyophilized powder or pre-mixed solution depending on the supplier. For lyophilized vials:

• Reconstitute with bacteriostatic water following standard peptide preparation procedures
• NAD+ solutions may appear slightly yellow — this is normal
• Inject slowly subcutaneously — NAD+ injections tend to sting more than typical peptide injections
• Some users dilute with additional BAC water to reduce concentration and injection discomfort

Use the peptide calculator for reconstitution volumes. For step-by-step preparation, see the reconstitution guide.

NAD+ dosing timing considerations:

• Time of day: Morning administration is commonly preferred, as NAD+ influences circadian clock gene expression and energy metabolism. Some users report insomnia with evening dosing
• Fasted vs. fed: No strict fasting requirement for injectable NAD+, unlike GH secretagogues
• Loading phase: Some protocols use a higher-frequency loading phase (daily for 2–4 weeks) followed by maintenance dosing (2–3× weekly)
• Cycle length: Protocols range from 4 weeks to ongoing supplementation. NAD+ levels begin to decline again after cessation

Unlike peptides that require cycling to prevent receptor desensitization, NAD+ is a metabolic substrate rather than a receptor agonist, so tolerance is not a primary concern.

NAD+ storage requirements:

• Lyophilized: Store at -20°C for long-term stability, or 2–8°C (refrigerator) for shorter periods
• Reconstituted: Refrigerate at 2–8°C. Use within 28 days
• NAD+ is light-sensitive — store in amber vials or protect from direct light
• Avoid repeated freeze-thaw cycles

For complete storage guidelines, see the peptide storage guide.