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MOTS-c: Complete Guide

MOTS-c (Mitochondrial Open Reading Frame of the Twelve S rRNA Type-C) is a mitochondrial-derived peptide consisting of 16 amino acids encoded within the mitochondrial genome. Discovered in 2015 by Dr. Changhan David Lee at the University of Southern California, MOTS-c has generated significant interest for its role in metabolic regulation, exercise mimetic effects, and potential anti-aging applications. It is the first mitochondrial-derived peptide shown to act as a signaling molecule regulating nuclear gene expression.

Last updated: 2026-01-28

Quick Facts

Category
therapeutic
Also Known As
Mitochondrial ORF of the Twelve S rRNA Type-C
Related Goals
fat loss, anti aging

Who Researches MOTS-c?

MOTS-c is researched by people interested in metabolic health, fat loss, and exercise performance — particularly those looking for a peptide that mimics some of the metabolic benefits of exercise. It's popular with people who want to improve insulin sensitivity, support body composition changes, or complement their fitness routine. MOTS-c is also studied in anti-aging contexts since levels decline naturally with age. If you're interested in metabolic optimization but don't want the appetite and GI effects of GLP-1 agonists like tirzepatide, MOTS-c targets a completely different pathway (AMPK activation).

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What Is MOTS-c?

MOTS-c represents a paradigm shift in understanding mitochondrial biology. Previously, mitochondrial DNA was thought to encode only proteins involved in oxidative phosphorylation (energy production). The discovery that mitochondria encode signaling peptides like MOTS-c that regulate nuclear gene expression opened an entirely new area of biology — mitonuclear communication.

MOTS-c is released from mitochondria into the cytoplasm and bloodstream, where it acts as a retrograde signal from mitochondria to the nucleus. During metabolic stress (such as exercise), MOTS-c translocates to the nucleus where it directly regulates gene expression related to metabolism, stress adaptation, and cellular homeostasis.

Mechanism of Action

  • AMPK activation: MOTS-c activates AMP-activated protein kinase, the master metabolic sensor that promotes glucose uptake, fat oxidation, and mitochondrial biogenesis
  • Folate metabolism: MOTS-c regulates the folate-methionine cycle, affecting de novo purine biosynthesis and one-carbon metabolism
  • Insulin sensitivity: Improves glucose disposal and insulin signaling in skeletal muscle
  • Exercise mimetic: Activates metabolic pathways normally stimulated by physical exercise, including AMPK-PGC1α signaling
  • Nuclear gene regulation: Translocates to the nucleus during stress to directly modulate gene expression related to metabolic adaptation

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Dosage Overview

MOTS-c research dosing (based on animal studies and early human research):

  • Subcutaneous: 5–10 mg daily or every other day
  • Research cycles: 4–8 weeks

Dosing is primarily extrapolated from animal studies (typically 5mg/kg in mice). Human clinical trials are underway but dosing guidelines remain preliminary. Use the peptide calculator for reconstitution.

Side Effects & Safety

As a naturally occurring peptide encoded by human mitochondrial DNA, MOTS-c is expected to have good biological compatibility. Limited safety data exists:

  • Injection site reactions: Mild redness reported
  • Blood glucose effects: As an insulin sensitizer, MOTS-c could theoretically cause hypoglycemia in combination with diabetes medications
  • Limited human data: Most evidence comes from animal studies and cell culture

Frequently Asked Questions

References

  1. Lee C, et al.. The mitochondrial-derived peptide MOTS-c promotes metabolic homeostasis and reduces obesity and insulin resistance. Cell Metabolism, 2015.
  2. Reynolds JC, et al.. MOTS-c is an exercise-induced mitochondrial-encoded regulator of age-dependent physical decline and muscle homeostasis. Nature Communications, 2021.

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Peptides Insider Editorial Team

Our content is reviewed for accuracy and grounded in peer-reviewed research where available. We do not provide medical advice. Always consult a qualified healthcare professional.