Thymosin Beta-4: Dosage & Administration
Part of the Thymosin Beta-4 Complete Guide
Research Peptides
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Thymosin Beta-4 Dosage Protocols
Thymosin beta-4 (Tβ4) dosing in research typically follows a loading and maintenance approach, similar to its fragment TB-500:
| Phase | Dose | Frequency | Duration |
|---|---|---|---|
| Loading | 750 mcg – 2 mg | Once or twice daily | 2–4 weeks |
| Maintenance | 750 mcg – 2 mg | 2–3× weekly | 4–8 weeks |
Thymosin beta-4 is the full-length 43-amino-acid peptide, while TB-500 is a synthetic fragment containing the active region. The full-length molecule has been more extensively studied in formal clinical trials, while TB-500 is more common in research settings due to cost and availability.
Reconstitution & Preparation
Thymosin beta-4 comes in lyophilized form:
- Reconstitute with bacteriostatic water following standard procedures
- Inject subcutaneously — abdomen or thigh
- The peptide acts systemically — injection location does not need to be near the injury site
Use the peptide calculator for volumes. See the reconstitution guide for preparation.
Timing & Cycle Length
Thymosin beta-4 timing:
- Time of day: No strict requirement. Any time of day is acceptable
- Total cycle: Loading (2–4 weeks) + maintenance (4–8 weeks) = 6–12 weeks total
- Injury-related use: Begin as soon as possible after injury for maximum benefit
- No fasting required
Storage
Standard peptide storage:
- Lyophilized: -20°C long-term, 2–8°C for months
- Reconstituted: Refrigerate at 2–8°C, use within 28 days
See the peptide storage guide.