Thymosin Beta-4: Dosage & Administration

Thymosin beta-4 (Tβ4) dosing in research typically follows a loading and maintenance approach, similar to its fragment TB-500:

Phase	Dose	Frequency	Duration
Loading	750 mcg – 2 mg	Once or twice daily	2–4 weeks
Maintenance	750 mcg – 2 mg	2–3× weekly	4–8 weeks

Thymosin beta-4 is the full-length 43-amino-acid peptide, while TB-500 is a synthetic fragment containing the active region. The full-length molecule has been more extensively studied in formal clinical trials, while TB-500 is more common in research settings due to cost and availability.

Thymosin beta-4 comes in lyophilized form:

• Reconstitute with bacteriostatic water following standard procedures
• Inject subcutaneously — abdomen or thigh
• The peptide acts systemically — injection location does not need to be near the injury site

Use the peptide calculator for volumes. See the reconstitution guide for preparation.

Thymosin beta-4 timing:

• Time of day: No strict requirement. Any time of day is acceptable
• Total cycle: Loading (2–4 weeks) + maintenance (4–8 weeks) = 6–12 weeks total
• Injury-related use: Begin as soon as possible after injury for maximum benefit
• No fasting required

Standard peptide storage:

• Lyophilized: -20°C long-term, 2–8°C for months
• Reconstituted: Refrigerate at 2–8°C, use within 28 days

See the peptide storage guide.