BPC-157 + TB-500 Stack: The Complete Healing Protocol
Published March 1, 2026
Why BPC-157 + TB-500 Is the Most Popular Healing Stack
The combination of BPC-157 and TB-500 (Thymosin Beta-4) is the most widely used peptide stack for healing. These compounds target complementary repair pathways. For the published research, see our BPC-157 + TB-500 Research article. For general stacking principles, see our Peptide Stacking Guide.
Complementary Mechanisms
BPC-157 and TB-500 cover different phases of tissue repair:[1]
| Pathway | BPC-157 | TB-500 |
|---|---|---|
| Angiogenesis | Strong (VEGF upregulation) | Moderate |
| Cell migration | Moderate | Strong (actin sequestration) |
| Inflammation | Moderate (NO system) | Strong (cytokine downregulation) |
| Growth factors | Strong (EGF, FGF, VEGF) | Moderate |
| Collagen synthesis | Moderate (tendon-specific) | Moderate (broad connective tissue) |
| Systemic vs local | Enhanced with local injection | Primarily systemic |
The Complete Dosing Protocol
Standard Healing Stack
| Compound | Dose | Frequency | Route |
|---|---|---|---|
| BPC-157 | 250-300 mcg | 2x daily | SubQ near injury |
| TB-500 | 2-2.5 mg | 2x per week | SubQ anywhere |
Unit warning: BPC-157 is dosed in micrograms (mcg). TB-500 in milligrams (mg). Do not confuse the units.
See the BPC-157 Dosage Guide for detailed rationale. Use the Reconstitution Calculator for volumes.
Loading Phase (Optional — Weeks 1-2)
- BPC-157: 500 mcg 2x daily
- TB-500: 5 mg 2x per week
Maintenance Phase (Weeks 2/3-8)
- BPC-157: 250 mcg 2x daily
- TB-500: 2 mg once per week
Injection Strategy
BPC-157: Inject subcutaneously near the injury site for local concentration advantages.[2] If impractical, systemic SubQ in the abdomen works.
TB-500: Works systemically — inject anywhere (abdomen, thigh, deltoid).
They can be injected at the same time but as separate injections. For technique, see the Injection Guide.
Cycle Structure
| Phase | Duration | BPC-157 | TB-500 |
|---|---|---|---|
| Loading | 1-2 weeks | 500 mcg 2x/day | 5 mg 2x/week |
| Maintenance | 4-6 weeks | 250 mcg 2x/day | 2 mg 1x/week |
| Total | 6-8 weeks | ||
| Off period | 4+ weeks |
See the Cycle Length Guide for duration recommendations across all peptides.
Reconstitution Quick Reference
BPC-157 (5 mg vial)
- Add 2 mL BAC water = 2,500 mcg/mL
- 250 mcg = 0.10 mL (10 units); 500 mcg = 0.20 mL (20 units)
TB-500 (5 mg vial)
- Add 1 mL BAC water = 5 mg/mL
- 2.5 mg = 0.50 mL (50 units)
Use bacteriostatic water for multi-dose vials.
Common Applications
- Tendon injuries (rotator cuff, Achilles, patellar)
- Ligament sprains (ACL, MCL)
- Muscle tears
- Post-surgical recovery
- Chronic pain conditions
For joint-specific applications, see the Joint Mobility Stack. For recovery with sleep support, see the Recovery Stack. Visit Healing for the complete overview.
Realistic Timeline
- Week 1-2: Reduced inflammation, initial pain improvement
- Week 2-4: Increased mobility and function
- Week 4-8: Structural healing progress (tendons/ligaments heal slowly)
All evidence is from animal models. These peptides should complement proper medical care and rehabilitation.
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Frequently Asked Questions
References
- Sikiric P, et al.. Stable gastric pentadecapeptide BPC 157: novel therapy in gastrointestinal tract. Current Pharmaceutical Design, 2011.
- Sosne G, et al.. Biological activities of thymosin beta-4 defined by active sites in short peptide sequences. FASEB Journal, 2010.
- Chang CH, et al.. Pentadecapeptide BPC 157 enhances the growth hormone receptor expression in tendon fibroblasts. Molecules, 2014.
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Peptides Insider Editorial Team
Our content is reviewed for accuracy and grounded in peer-reviewed research where available. We do not provide medical advice. Always consult a qualified healthcare professional.